产品简介
beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.
产品性质
别名:ACTB;Actin; cytoplasmic 1; Beta-actin; Beta actin; BRWS1; Actin; beta; Beta cytoskeletal actin; PS1TP5-binding protein 1; PS1TP5BP1;
蛋白号(Uniprot):P60709
Isotype:IgG
Purificationmethod:Affinity purification
反应:Human,Mouse,Rat,Pig,Zebrafish,Bovine,Sheep,Rabbit,Goat,Guineapig,Dog,Monkey,Hamster,Duck,Chicken,Fish
应用(Application):WB, IF, IHC, ICC
来源(Source):Rabbit
克隆(Clonality):Polyclonal
理论分子量(Mol.Wt): 43kDa; 42kD(Calculated).
浓度:: ~1mg/ml
偶联:Unconjugated.
保存条件(Storage):Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
产品应用(实际使用时需根据抗原水平的高低作适当调整)
建议稀释浓度:
WB 1:5000-1:20000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
注意事项
1)为了您的安全和健康,请穿实验服并戴一次性手套操作。
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